Figure 2
Ferrous iron-induced generation of hydroxyl radicals in the presence of 10 μM APF and 50 mM phosphate pH 7.40 buffer through the Fe/O 2 and Fenton reactions at room temperature (22 ± 2°C). Ferrous iron was added as a sulfate salt. The experiment was repeated in the absence of molecular oxygen. The hROS reactivity values can be compared to the reported fluorescence intensity values shown on the other figures by dividing hROS reactivity by ten.