Figure 1

(A) Adenine degradation by Fenton-generated hydroxyl radicals. 100 μM adenine was mixed with either Fe(II), H₂O₂ or a combination of the two, which generates ^•OH. The solutions were incubated for 24 hrs followed by wavelength scans. Loss of absorbance at 260 nm is indicative of degradation of the adenine molecule. (B) 64 kUnits catalase or 50% ethanol were added to adenine solutions with Fenton reagents. The 1 mM H₂O₂ & 1 mM Fe(II) plot from graph A is included for comparison. Note that the order in the legend follows the same order of curves in the graphs from top to bottom so in (A), the top three curves overlap (i.e., "nothing added", "1 mM Fe(II)", and "1000 μM H₂O₂").