Phenylalanine as a hydroxyl radical-specific probe in pyrite slurries

Table 1 Experimental Design

Experiment Set	[Phe]_o	[pyr]	Incubation time	pH
	(μM)	(g/L)	(hr)
A	26.6	100		2.5
	102.8	100	72	2.6
	306.7	100		2.5
B	12.5	100
	37.1	100
	97.8	100	120
	401.5	100
	1127	100
C	1	100
	3	100	6-hour pyrite incubations + 1 hr pyrite and Phe
	10	100
	30	100
	100	100
D	10.9	25
	11.4	50
	11.4	100
	28.5	25
	29.5	50
	28.6	100	12
	93.9	25
	91.9	50
	94.5	100
	285.2	25
	281.4	50
	285.6	100
E	8.7	5		5.5
	9.4	10		5.1
	9.3	25	16	4.2
	10.4	50		2.9
	10.4	100		2.6
F	81.68	10	72
	107.2	10 (14 m ² /g)	240
G	98.8	50		2.8
	[o-Tyr]_o 94.0	50	72
	[m-Tyr]_o 97.6	50
	[p-Tyr]_o 97.7	50

Experiments A - G listed above with corresponding [Phe]_o, initial pyrite loading, incubation time of the experiment, and pH data. The pH was not monitored in every reaction vessel for each experiment as it was not a variable as all (Exp. B, C, F, and G) or multiple vials (Exp. D) had the same pyrite loadings. Reaction vessels in experiment G contained only one reactant each - Phe, o-Tyr, m-Tyr, or p-Tyr. Pyrite was added to the respective dilutions of Phe (or Tyr) to initiate the reaction.

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