Table IV List of 16S rRNA gene sequences and their taxonomic affiliations (based on BLAST searches of NCBI GenBank database) from the January 19, 2001 sampling. n.i. = not included in phylogenetic analyses.
From: Acid mine drainage biogeochemistry at Iron Mountain, California
Mine site | Clones in Fig. 7 | Closest match | Similarity | Classification | No. of clones |
|---|---|---|---|---|---|
01IM1-A drift "red pool" | n.i. | Acidithiobacillus ferrooxidans | 99% | Gammaproteobacteria | 21 |
| Â | BA33 | Bond clone BA29 (Leptosprillum group III) | 97% | Nitrospira | 7 |
| Â | BA24 | Leptospirillum ferriphilum | Â | Nitrospira | 4 |
| Â | BA39 | Leptospirillum ferrooxidans strain 49879 | 99% | Nitrospira | 3 |
| Â | BA8 | Sulfobacillus sp. Frasl, AF213055 | 94% | Bacillales | 3 |
| Â | BA9, BA31, BA71 | Iron Mountain Bond clone BA71 | 99% | Deltaproteobacteria | 3 |
| Â | BA2 | Uncultured eubacterium WD247, AJ292581 | 95% | Acidobacteria | 1 |
A drift slump | AS 10 | Uncultured coal refuse clone ARCP1-21 | 96% | B plasma, Thermoplasmales | 6 |
| Â | AS1, AS7 | Uncultured coal refuse clone ARCP1-21 | 93% and 95% | Cplasma, Thermoplasmales | 5 |
| Â | AS4 | Uncultured coal refuse clone ARCP1-27 | 98% | Dplasma, Thermoplasmales | 4 |
| Â | AS9 | Bond clone BA29 (Leptosprillum group III) | 98% | Nitrospira | 7 |
| Â | AS6 | Iron Mountain Bond clone BA18 | 98% | Deltaproteobacteria | 3 |
| Â | n.i. | Iron Mountain Bond clone BA71 | 99% | Deltaproteobacteria | 1 |
| Â | n.i. | Leptospirillum ferriphilum | 97% | Nitrospira | 2 |
| Â | Several (Ref. 38) | Ferroplasma acidarmanus | >98% | Thermoplasmales | 1 |
A drift slime streamers | Several (Ref. 38) | Ferroplasma acidarmanus | >98% | Thermoplasmales | 20 |
| Â | ASL9 | Bond clone BA29 (Leptosprillum group III) | 98% | Nitrospira | 18 |
| Â | ASL1, ASL32 | Uncultured coal refuse clone ARCP1-28 | 97% and 98% | Aplasma, Thermoplasmales | 8 |
| Â | ASL8 | Rheims clone TM214 | 91% | Actinobacteria | 3 |
| Â | ASM | Ferromicrobium acidophilum | 98% | Actinobacteria | 1 |
| Â | Baker et al. (Ref. 52) | Endosymbiont of Acanthamoeba sp. TUMK23 | 92% | Alphaproteobacteria | 2 |
01IMA1 (A drift weir) | Several (Ref. 38) | Ferroplasma acidarmanus | >98% | Thermoplasmales | 14 |
| Â | AW1, AW4 | Bond clone BA29 (Leptosprillum group III) | 98% and 99% | Nitrospira | 13 |
| Â | Baker et al. (Ref. 52) | Endosymbiont of Acanthamoeba sp. TUMK23 | 92% | Alphaproteobacteria | 2 |
| Â | n.i. | Sulfobacillus sp. Frasl, AF213055 | 94% | Bacillales | 1 |
01IM1B1 (B drift weir) | Several (Ref. 38) | Ferroplasma acidarmanus | >98% | Thermoplasmales | 26 |
| Â | n.i. | Bond clone BA29 (Leptosprillum group III) | 98% | Nitrospira | 21 |
| Â | n.i. | Sulfobacillus sp. Frasl, AF213055 | 94% | Bacillales | 2 |
| Â | BW7 | Sulfobacillus disulfoxidans SD-11, U34974 | 99% | Bacillales | 1 |
01IM1C1 (C drift weir) | n.i. | Bond clone BA29 (Leptosprillum group III) | 98% | Nitrospira | 18 |
| Â | Several (Ref. 38) | Ferroplasma acidarmanus | >98% | Thermoplasmales | 13 |
| Â | n.i. | Sulfobacillus sp. Frasl, AF213055 | 94% | Bacillales | 5 |