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Table I Experimental conditions for batch cultures

From: Secondary mineral formation associated with respiration of nontronite, NAu-1 by iron reducing bacteria

 

Initial Conditions

Auqueous phase Fe results

Solid Fe extraction results

Fe reduction

Sample ID

Description

Run (hrs)

Total NAu-1 in system (g)

Fe(III) (mols) in initial NAu-1

Aqueous culture media (25% Ml) (ml)

Fe(total) (mols) in initial aqueous culture media

Total Fe(aq) in mediaa (M)

% Fe in system partitioned to aqueous phase

Fe(II) in mediab (M)

Fe(II)+ Fe(III) in mediab (M)

Fe(III) in media by difference (M)

Fe(II) in aqueous media (mols)

Extraction fluid volumec (ml)

Fe(II) in extraction fluid (M)

Fe(II) in extraction fluid (mols)

Total reduced Fe(II)d (micromols)

% Fe reduction

200

Anoxic Live

4

0.0403

1.81E-04

10

1.35E-08

1.68E-06

0.0

    

20

1.96E-05

3.92E-07

3.92E-07

0.2

201

Anoxic Live

4

0.0401

1.80E-04

10

1.35E-08

1.45E-06

0.0

    

20

1.32E-05

2.63E-07

2.63E-07

01

202

Anoxic Live

4

0.0401

1.80E-04

10

1.35E-08

1.51E-06

0.0

    

20

    

203

Anoxic Live

4

0.0402

1.81E-04

10

1.35E-08

1.75E-06

0.0

    

20

    

204

Anoxic Live

24

0.0401

1.80E-04

10

1.35E-08

5.72E-06

0.0

6.00E-07

3.68E-06

3.08E-06

6.00E-09

20

5.19E-05

1.04E-06

1.04E-06

0.6

205

Anoxic Live

24

0.0402

1.81E-04

10

1.35E-08

8.98E-06

0.0

    

20

5.19E-05

1.04E-06

1.04E-06

0.6

206

Anoxic Live

24

0.0402

1.81E-04

10

1.35E-08

8.44E-06

0.0

    

20

    

207

Anoxic Live

24

0.0400

1.80E-04

10

1.35E-08

8.52E-06

0.0

    

20

    

208

Anoxic Live

48

0.0399

1.80E-04

10

1.35E-08

1.31E-05

0.1

8.00E-07

1.62E-05

1.54E-05

8.00E-09

20

1.49E-04

2.97E-06

2.98E-06

17

209

Anoxic Live

48

0.0402

1.81E-04

10

1.35E-08

1.41E-05

0.1

    

20

2.00E-04

4.00E-06

4.00E-06

2.2

210

Anoxic Live

48

0.0400

1.80E-04

10

1.35E-08

1.36E-05

0.1

    

20

    

211

Anoxic Live

48

0.0400

1.80E-04

10

1.35E-08

1.37E-05

0.1

    

20

    

212

Anoxic Live

168

0.0400

1.80E-04

10

1.35E-08

1.85E-05

0.1

6.00E-07

1.84E-05

1.78E-05

6.00E-09

20

3.94E-04

7.87E-06

7.888E-06

4.4

213

Anoxic Live

168

0.0403

1.81E-04

10

1.35E-08

2.27E-05

0.1

    

20

4.20E-04

8.39E-06

8.39E-06

4.6

214

Anoxic Live

168

0.0399

1.80E-04

10

1.35E-08

2.02E-05

0.1

    

20

    

215

Anoxic Live

168

0.0400

1.80E-04

10

1.35E-08

7.10E-06

0.0

7.90E-06

1.35E-05

5.59E-06

7.90E-08

20

    

216

Anoxic Killed

4

0.0403

1.81E-04

10

1.35E-08

1.62E-06

0.0

    

20

6.70E-06

1.34E-07

1.34E-07

0.1

217

Anoxic Killed

4

0.0403

1.81E-04

10

1.35E-08

1.63E-06

0.0

    

20

6.70E-06

1.34E-07

1.34E-07

0.1

218

Anoxic Killed

4

0.0399

1.80E-04

10

1.35E-08

1.60E-06

0.0

    

20

    

219

Anoxic Killed

24

0.0401

1.80E-04

10

1.35E-08

1.80E-06

0.0

BDLe

1.48E-06

1.34E-06

BDL

20

6.70E-06

1.34E-07

1.33E-07

0.1

220

Anoxic Killed

24

0.0402

1.81E-04

10

1.35E-08

1.79E-06

0.0

    

20

1.32E-05

2.63E-07

2.63E-07

0.1

221

Anoxic Killed

24

0.0403

1.81E-04

10

1.35E-08

1.86E-06

0.0

    

20

    

222

Anoxic Killed

48

0.0402

1.81E-04

10

1.35E-08

2.41E-06

0.0

BDL

1.42E-06

1.42E-06

BDL

20

6.70E-06

1.34E-07

1.34E-07

0.1

223

Anoxic Killed

48

0.0401

1.80E-04

10

1.35E-08

2.51E-06

0.0

    

20

1.96E-05

3.92E-07

3.92E-07

0.2

224

Anoxic Killed

48

0.0400

1.80E-04

10

1.35E-08

2.47E-06

0.0

    

20

    

225

Anoxic Killed

168

0.0399

1.80E-04

10

1.35E-08

1.33E-05

0.1

3.80E-06

2.01E-05

1.63E-05

3.80E-08

20

1.03E-04

2.07E-06

2.10E-06

1.2

226

Anoxic Killed

168

0.0400

1.80E-04

10

1.35E-08

8.71E-06

0.0

    

20

2.61E-05

5.21E-07

5.21E-07

0.3

227

Anoxic Killed

168

0.0403

1.81E-04

10

1.35E-08

1.89E-05

0.1

    

20

    

228

Oxic Live

4

0.0401

1.80E-04

10

1.35E-08

1.32E-05

0.1

    

20

3.25E-05

6.50E-07

6.50E-07

0.4

229

Oxic Live

4

0.0400

1.80E-04

10

1.35E-08

1.20E-05

0.1

    

20

1.32E-05

2.63E-07

2.63E-07

0.1

230

Oxic Live

4

0.0402

1.81E-04

10

1.35E-08

1.27E-05

0.1

    

20

    

231

Oxic Live

24

0.0400

1.80E-04

10

1.35E-08

1.68E-05

0.1

1.10E-06

1.34E-05

1.23E-05

1.10E-08

20

3.25E-05

6.50E-07

6.58E-07

0.4

232

Oxic Live

24

0.0402

1.81E-04

10

1.35E-08

1.54E-05

0.1

BDL

1.59E-05

1.59E-05

BDL

20

2.50E-07

5.00E-09

5.00E-09

0.0

233

Oxic Live

24

0.0399

1.80E-04

10

1.35E-08

1.56E-05

0.1

    

20

    

234

Oxic Live

48

0.0399

1.80E-04

10

1.35E-08

1.75E-06

0.0

    

20

2.50E-07

5.00E-09

5.00E-09

0.0

235

Oxic Live

48

0.0401

1.80E-04

10

1.35E-08

1.60E-05

0.1

BDL

1.56E-05

1.56E-05

BDL

20

1.96E-05

3.92E-07

3.92E-07

0.2

236

Oxic Live

48

0.0399

1.80E-04

10

1.35E-08

1.59E-05

0.1

    

20

    

237

Oxic Live

168

0.0399

1.80E-04

10

1.35E-08

1.86E-05

0.1

7.00E-07

1.64E-05

1.57E-05

7.00E-09

20

4.54E-05

9.08E-07

9.13E-07

0.5

238

Oxic Live

168

0.0399

1.80E-04

10

1.35E-08

1.887E-05

0.1

    

20

6.70E-06

1.34E-07

1.34E-07

0.1

239

Oxic Live

168

0.0401

1.80E-04

10

1.35E-08

1.91E-05

0.1

    

20

    
  1. aBy ICP.
  2. bBy ferrozine assay.
  3. cIncludes 3 ml supernatant and 17 ml 0.6 N HC1.
  4. dSum of Fe(II) in 0.5 N HC1 extraction solution and dissolved Fe(II) that was removed when 7 ml supernatant was removed.
  5. eDetection limit of 0.4 μM was assumed after Washington et al. (Ref. 26).
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Geochemical Transactions

ISSN: 1467-4866

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