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Table I Experimental conditions for batch cultures

From: Secondary mineral formation associated with respiration of nontronite, NAu-1 by iron reducing bacteria

  Initial Conditions Auqueous phase Fe results Solid Fe extraction results Fe reduction
Sample ID Description Run (hrs) Total NAu-1 in system (g) Fe(III) (mols) in initial NAu-1 Aqueous culture media (25% Ml) (ml) Fe(total) (mols) in initial aqueous culture media Total Fe(aq) in mediaa (M) % Fe in system partitioned to aqueous phase Fe(II) in mediab (M) Fe(II)+ Fe(III) in mediab (M) Fe(III) in media by difference (M) Fe(II) in aqueous media (mols) Extraction fluid volumec (ml) Fe(II) in extraction fluid (M) Fe(II) in extraction fluid (mols) Total reduced Fe(II)d (micromols) % Fe reduction
200 Anoxic Live 4 0.0403 1.81E-04 10 1.35E-08 1.68E-06 0.0      20 1.96E-05 3.92E-07 3.92E-07 0.2
201 Anoxic Live 4 0.0401 1.80E-04 10 1.35E-08 1.45E-06 0.0      20 1.32E-05 2.63E-07 2.63E-07 01
202 Anoxic Live 4 0.0401 1.80E-04 10 1.35E-08 1.51E-06 0.0      20     
203 Anoxic Live 4 0.0402 1.81E-04 10 1.35E-08 1.75E-06 0.0      20     
204 Anoxic Live 24 0.0401 1.80E-04 10 1.35E-08 5.72E-06 0.0 6.00E-07 3.68E-06 3.08E-06 6.00E-09 20 5.19E-05 1.04E-06 1.04E-06 0.6
205 Anoxic Live 24 0.0402 1.81E-04 10 1.35E-08 8.98E-06 0.0      20 5.19E-05 1.04E-06 1.04E-06 0.6
206 Anoxic Live 24 0.0402 1.81E-04 10 1.35E-08 8.44E-06 0.0      20     
207 Anoxic Live 24 0.0400 1.80E-04 10 1.35E-08 8.52E-06 0.0      20     
208 Anoxic Live 48 0.0399 1.80E-04 10 1.35E-08 1.31E-05 0.1 8.00E-07 1.62E-05 1.54E-05 8.00E-09 20 1.49E-04 2.97E-06 2.98E-06 17
209 Anoxic Live 48 0.0402 1.81E-04 10 1.35E-08 1.41E-05 0.1      20 2.00E-04 4.00E-06 4.00E-06 2.2
210 Anoxic Live 48 0.0400 1.80E-04 10 1.35E-08 1.36E-05 0.1      20     
211 Anoxic Live 48 0.0400 1.80E-04 10 1.35E-08 1.37E-05 0.1      20     
212 Anoxic Live 168 0.0400 1.80E-04 10 1.35E-08 1.85E-05 0.1 6.00E-07 1.84E-05 1.78E-05 6.00E-09 20 3.94E-04 7.87E-06 7.888E-06 4.4
213 Anoxic Live 168 0.0403 1.81E-04 10 1.35E-08 2.27E-05 0.1      20 4.20E-04 8.39E-06 8.39E-06 4.6
214 Anoxic Live 168 0.0399 1.80E-04 10 1.35E-08 2.02E-05 0.1      20     
215 Anoxic Live 168 0.0400 1.80E-04 10 1.35E-08 7.10E-06 0.0 7.90E-06 1.35E-05 5.59E-06 7.90E-08 20     
216 Anoxic Killed 4 0.0403 1.81E-04 10 1.35E-08 1.62E-06 0.0      20 6.70E-06 1.34E-07 1.34E-07 0.1
217 Anoxic Killed 4 0.0403 1.81E-04 10 1.35E-08 1.63E-06 0.0      20 6.70E-06 1.34E-07 1.34E-07 0.1
218 Anoxic Killed 4 0.0399 1.80E-04 10 1.35E-08 1.60E-06 0.0      20     
219 Anoxic Killed 24 0.0401 1.80E-04 10 1.35E-08 1.80E-06 0.0 BDLe 1.48E-06 1.34E-06 BDL 20 6.70E-06 1.34E-07 1.33E-07 0.1
220 Anoxic Killed 24 0.0402 1.81E-04 10 1.35E-08 1.79E-06 0.0      20 1.32E-05 2.63E-07 2.63E-07 0.1
221 Anoxic Killed 24 0.0403 1.81E-04 10 1.35E-08 1.86E-06 0.0      20     
222 Anoxic Killed 48 0.0402 1.81E-04 10 1.35E-08 2.41E-06 0.0 BDL 1.42E-06 1.42E-06 BDL 20 6.70E-06 1.34E-07 1.34E-07 0.1
223 Anoxic Killed 48 0.0401 1.80E-04 10 1.35E-08 2.51E-06 0.0      20 1.96E-05 3.92E-07 3.92E-07 0.2
224 Anoxic Killed 48 0.0400 1.80E-04 10 1.35E-08 2.47E-06 0.0      20     
225 Anoxic Killed 168 0.0399 1.80E-04 10 1.35E-08 1.33E-05 0.1 3.80E-06 2.01E-05 1.63E-05 3.80E-08 20 1.03E-04 2.07E-06 2.10E-06 1.2
226 Anoxic Killed 168 0.0400 1.80E-04 10 1.35E-08 8.71E-06 0.0      20 2.61E-05 5.21E-07 5.21E-07 0.3
227 Anoxic Killed 168 0.0403 1.81E-04 10 1.35E-08 1.89E-05 0.1      20     
228 Oxic Live 4 0.0401 1.80E-04 10 1.35E-08 1.32E-05 0.1      20 3.25E-05 6.50E-07 6.50E-07 0.4
229 Oxic Live 4 0.0400 1.80E-04 10 1.35E-08 1.20E-05 0.1      20 1.32E-05 2.63E-07 2.63E-07 0.1
230 Oxic Live 4 0.0402 1.81E-04 10 1.35E-08 1.27E-05 0.1      20     
231 Oxic Live 24 0.0400 1.80E-04 10 1.35E-08 1.68E-05 0.1 1.10E-06 1.34E-05 1.23E-05 1.10E-08 20 3.25E-05 6.50E-07 6.58E-07 0.4
232 Oxic Live 24 0.0402 1.81E-04 10 1.35E-08 1.54E-05 0.1 BDL 1.59E-05 1.59E-05 BDL 20 2.50E-07 5.00E-09 5.00E-09 0.0
233 Oxic Live 24 0.0399 1.80E-04 10 1.35E-08 1.56E-05 0.1      20     
234 Oxic Live 48 0.0399 1.80E-04 10 1.35E-08 1.75E-06 0.0      20 2.50E-07 5.00E-09 5.00E-09 0.0
235 Oxic Live 48 0.0401 1.80E-04 10 1.35E-08 1.60E-05 0.1 BDL 1.56E-05 1.56E-05 BDL 20 1.96E-05 3.92E-07 3.92E-07 0.2
236 Oxic Live 48 0.0399 1.80E-04 10 1.35E-08 1.59E-05 0.1      20     
237 Oxic Live 168 0.0399 1.80E-04 10 1.35E-08 1.86E-05 0.1 7.00E-07 1.64E-05 1.57E-05 7.00E-09 20 4.54E-05 9.08E-07 9.13E-07 0.5
238 Oxic Live 168 0.0399 1.80E-04 10 1.35E-08 1.887E-05 0.1      20 6.70E-06 1.34E-07 1.34E-07 0.1
239 Oxic Live 168 0.0401 1.80E-04 10 1.35E-08 1.91E-05 0.1      20     
  1. aBy ICP.
  2. bBy ferrozine assay.
  3. cIncludes 3 ml supernatant and 17 ml 0.6 N HC1.
  4. dSum of Fe(II) in 0.5 N HC1 extraction solution and dissolved Fe(II) that was removed when 7 ml supernatant was removed.
  5. eDetection limit of 0.4 μM was assumed after Washington et al. (Ref. 26).