Pyrite-induced hydroxyl radical formation and its effect on nucleic acids

Table 1 H₂O₂ Measurements*

Pyrite loading (g/L)^a	H₂O₂(μM)
20 g/L with 1 mM EDTA	2.19
40 g/L with 1 mM EDTA	6.54
80 g/L with 10 mM EDTA	11.7
160 g/L with 10 mM EDTA	25.6
no EDTA added to any above	not detected
with EDTA and addition of catalase^b	not detected
Solution composition	H ₂ O ₂ (μM)
10 μM H₂O₂ solution no pyrite	10
with 10 μM Fe(II)	4.2
with 1 mM EDTA	10.1
with Fe(II) and EDTA	10.1

*Experiments were performed in the presence of oxygen. ^aPyrite of varying particle loadings were suspended in EDTA, quickly filtered and analysed.
^b127,000 units catalase/4 mL.

ISSN: 1467-4866