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Table 2 Results of phospholipid fatty acid (PLFA) analysis of Penn Mine sediment.

From: Microbial sulfate reduction and metal attenuation in pH 4 acid mine water

Total biomass

35,100 pmoles g-1

Cell equivalent value1

7.08 × 108

Bacterial biomass

31,900 pmoles g-1

Eukaryotic biomass

3,230 pmoles g-1

ratio bacteria/eukarya

10

Community Structure

% PLFA

Gram+/anaerobic Gram- (TerBrSats)

9.0

Gram- (Monoenoic)

36.8

Anaerobic metal reducers (Branched monoenoic)

0.0

SRB (Mid-chain branched)

18.2

Genera (Normal saturates)

26.8

Eukaryotes (polyenoics)

9.2

  1. 1 The cell equivalent value is calculated from experiments with typical bacteria isolated from soil and water [62]. This value is based on 2.0 × 1012 cells per gram dry weight of cells and 108 picomoles of phospholipid per gram dry weight of cells. The number of cells per gram of dry weight may vary and is dependent on the environmental conditions from which the microorganisms were recovered.