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Table 4 Sequence results from bands excised from DGGE of experimental cultures (Figure 6). Sequence identifications were performed using the BLASTN facility of the National Center for Biotechnology Information [47]. Similarity indices above 90% are considered excellent, 70–80% are good, and below 60% are considered to be unique sequences.

From: Microbial sulfate reduction and metal attenuation in pH 4 acid mine water

Band

Closest Match

Similarity Index

Phylogemetoc Affiliation

F

Bacillus sp. uncultured soil bacterium

100%

Bacillaceae

G, K

Desulfosporosinus sp. uncultured bacterium

92%

92%

Clostridia

H

Desulfitobacterium sp.

83%

Clostridia

I

uncultured bacterium

71%

Clostridia

J

uncultured bacterium

61%

Clostridia

L

Burkholderia sp. Ralstonia sp.

78%

Proteobacteria – Burkholderiaceae