Calculation of the relative metastabilities of proteins using the CHNOSZ software package

Table 1 Model proteins used in the present study.

Organism	T _opt ^a	Protein^b	ID	Length	Formula^c	$Δ G_{f}^{\circ c}$
Methanothermus sociabilis	88 [62]	CSG_METSC	P27374	571	C₂₈₁₂H₄₄₀₅N₇₄₇O₈₇₂S₁₆	-21875
Methanocaldococcus jannaschii	85 [43]	CSG_METJA	Q58232	530	C₂₅₅₅H₄₀₃₂N₆₄₀O₈₆₅S₁₄	-24236
Methanothermus fervidus	83 [63]	CSG_METFE	P27373	571	C₂₈₁₅H₄₄₁₁N₇₄₇O₈₇₂S₁₄	-21796
Haloarcula japonica	42 [64]	CSG_HALJP	Q9C4B4	828	C₃₆₆₉H₅₆₄₇N₉₇₁O₁₄₈₈	-43458
Methanococcus voltae	38 [42]	CSG_METVO	Q50833	553	C₂₅₇₅H₄₀₉₇N₆₄₅O₈₈₄S₁₁	-24881
Methanococcoides burtonii	23 [65]	CSG_METBU	Q12YZ7	278	C₁₃₆₂H₂₁₁₁N₃₅₅O₄₄₂S₄	-11677
Acetogenium kivui	66 [66]	SLAP_ACEKI	P22258	736	C₃₅₈₄H₅₆₄₈N₉₂₆O₁₁₃₈S	-29331
Bacillus stearothermophilus	65 [67]	SLAP_BACST	P35825	1198	C₅₆₇₆H₉₁₁₃N₁₄₈₉O₁₈₆₃S₃	-48792
Bacillus licheniformis	50 [68]	SLAP_BACLI	P49052	844	C₃₉₇₇H₆₃₉₆N₁₀₆₈O₁₂₈₆S₂	-33598
Aeromonas salmonicida	23 [69]	SLAP_AERSA	P35823	481	C₂₂₅₀H₃₅₈₀N₆₁₈O₇₁₆S₂	-18233

a. T_opt stands for laboratory optimal growth temperatures in °C, which were taken from the indicated sources. b. Compositions of cell-surface glycoproteins (CSG) from archaea and surface-layer proteins (SLAP) from bacteria were taken from the Swiss-Prot/UniProt database [37] using the noted accession numbers (IDs). Signal sequences were excluded where annotated, and names of the proteins except CSG_METBU correspond to those used in the Swiss-Prot database. c. Chemical formulas and standard molal Gibbs energies of formation from the elements at 25°C and 1 bar ( $Δ G_{f}^{\circ}$ , in kcal mol^-1) were calculated for nonionized aqueous proteins.

ISSN: 1467-4866